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Dipartimento di Biologia Animale ed Ecologia Marina, Università di Messina, Salita Sperone, 31, Messina, Italy
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- PublicationRestrictedBacterial and archaeal populations at two shallow hydrothermal vents off Panarea Island (Eolian Islands, Italy)(2009-01)
; ; ; ; ; ; ;Maugeri, T.; Dipartimento di Biologia Animale ed Ecologia Marina, Universita` di Messina ;Lentini, V.; Dipartimento di Biologia Animale ed Ecologia Marina, Universita` di Messina ;Gugliandolo, C.; Dipartimento di Biologia Animale ed Ecologia Marina, Universita` di Messina ;Italiano, F.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Palermo, Palermo, Italia ;Cousin, S.; Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, ;Stackebrandt, E.; ; ; ; ; The aim of this study was to investigate the microbial community thriving at two shallow hydrothermal vents off Panarea Island (Italy). Physico-chemical characteristics of thermal waters were examined in order to establish the effect of the vents on biodiversity of both Bacteria and Archaea. Water and adjacent sediment samples were collected at different times from two vents, characterised by different depth and temperature, and analysed to evaluate total microbial abundances, sulphuroxidising and thermophilic aerobic bacteria. Total microbial abundances were on average of the order of 105 cells ml-1, expressed as picoplanktonic size fraction. Picophytoplanktonic cells accounted for 0.77–3.83% of the total picoplanktonic cells. The contribution of bacterial and archaeal taxa to prokaryotic community diversity was investigated by PCR–DGGE fingerprinting method. The number of bands derived from bacterial DNA was highest in the DGGE profiles of water sample from the warmest and deepest site (site 2). In contrast, archaeal richness was highest in the water of the coldest and shallowest site (site 1). Sulphur-oxidising bacteria were detected by both culture- dependent and -independent methods. The primary production at the shallow hydrothermal system of Panarea is supported by a complex microbial community composed by phototrophs and chemolithotrophs.390 28 - PublicationOpen AccessThe submarine hydrothermal system of Panarea (Southern Italy): biogeochemical processes at the thermal fluids - sea bottom interface(2006-04-06)
; ; ; ;Gugliandolo, C.; Dipartimento di Biologia Animale ed Ecologia Marina, Università di Messina, Salita Sperone, 31, Messina, Italy ;Italiano, F.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Palermo, Palermo, Italia ;Maugeri, T.; Dipartimento di Biologia Animale ed Ecologia Marina, Università di Messina, Salita Sperone, 31, Messina, Italy; ; Among the submarine hydrothermal systems located offshore the volcanic archipelago of the Aeolian Islands (Southern Italy), the most active is located off the coasts of Panarea island. Thermal waters, gases and sulfur deposits coexist at the sea bottom where hydrothermal fluids are released from both shallow and deep vents. The chemical and isotopic composition of the fluid phase shows the presence of a significant magmatic component and the physico-chemical conditions of the geothermal reservoir allow the release of reduced chemical species that are microbially mediated towards the production of organic carbon as a form of biochemical energy. Microorganisms inhabiting this environment possess nutritional requirements and overall metabolic pathways ideally suited to such ecosystem that represents a clear example of the close connection between geosphere and biosphere. Microscopic examination of the white mat attached to rock surfaces showed the presence of Thiothrix-like filamentous bacteria. Moderately thermophilic heterotrophic isolates were identified as strains of the genus Bacillus. Although the hydrothermal system of Panarea has to be considered a “shallow” system, it shows many characteristics that make it similar to the “deep” oceanic systems, giving a unique opportunity for improving our knowledge on such an unexplored world by working at this easily accessible site.276 554 - PublicationRestrictedChanges in prokaryotic community composition accompanying a pronounced temperature shift of a shallow marine thermal brine pool (Panarea Island, Italy)(2015-01)
; ; ; ; ; ; ;Gugliandolo, C.; Department of Biological and Environmental Sciences, Research Centre for Extreme Environments and Extremophiles, University of Messina, ;Lentini, V.; Department of Biological and Environmental Sciences, Research Centre for Extreme Environments and Extremophiles, University of Messina, ;Bunk, B.; Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, ;Overmann, J.; Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen ;Italiano, F.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Palermo, Palermo, Italia ;Maugeri, T.; Department of Biological and Environmental Sciences, Research Centre for Extreme Environments and Extremophiles, University of Messina,; ; ; ; ; Hot Lake is a recently described thermal brine pool off Panarea Island (Eolian Islands, Italy) where emitted fluids are highly saline and rich in CO2 and H2S. The prokaryotic community composition in surface sediment samples was analyzed by high-throughput Illumina sequencing targeting the V3 region of the 16S rRNA at two time points that differed mainly with respect to temperature conditions, high-temperature (94 °C, HT09) and low-temperature (28.5 °C, LT10). Bacterial richness and diversity were greater than those of Archaea under both temperature conditions. In contrast to Bacteria, diversity and evenness of Archaea greatly increased at LT10. While the phylogenetic analysis indicated the presence of members mostly affiliated with the same taxonomic groups, their relative abundances differed from HT09 to LT10, resulting in different bacterial and archaeal assemblages. Both HT09 and LT10 were dominated by members of the Epsilonproteobacteria. Within this subphylum, bacteria of the genus Sulfurimonas were most frequently detected at HT09, while Arcobacter prevailed at LT10. The abundance of other dominant taxonomic groups (≥1 % of Illumina reads) also correlated with temperature conditions. Members assigned to hyperthermophilic Euryarchaeota (Thermococci) or to thermophilic (Caldiserica) and thermoresistant (Firmicutes) bacterial taxa were dominant at HT09, while those related to non-thermophilic Bacteroidetes, Fusobacteria and Actinobacteria were dominant at LT10. Several, probably photosynthetic, members of the Alphaproteobacteria, Chlorobi, Cyanobacteria and Chloroflexi were recovered under both temperature conditions. The co-occurrence of photosynthetic and chemolithotrophic microorganisms represents a unique feature of shallow vents such as Hot Lake260 63