Please use this identifier to cite or link to this item:
DC FieldValueLanguage
dc.contributor.authorallGagliano, A. L.; Università di Palermo, Dip. DiSTeMen
dc.contributor.authorallD'Alessandro, W.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Palermo, Palermo, Italiaen
dc.contributor.authorallTagliavia, M.; Università di Palermo, Dip. STEBICEFen
dc.contributor.authorallParello, F.; Università di Palermo, Dip. DiSTeMen
dc.contributor.authorallQuatrini, P.; Università di Palermo, Dip. STEBICEFen
dc.description.abstractVolcanic and geothermal systems emit endogenous gases by widespread degassing from soils, including CH4, a greenhouse gas twenty-five times as potent as CO2. Recently, it has been demonstrated that volcanic or geothermal soils are not only a source of methane, but are also sites of methanotrophic activity. Methanotrophs are able to consume 10–40 Tg of CH4 a−1 and to trap more than 50% of the methane degassing through the soils. We report on methane microbial oxidation in the geothermally most active site of Pantelleria (Italy), Favara Grande, whose total methane emission was previously estimated at about 2.5Mga−1 (t a−1). Laboratory incubation experiments with three top-soil samples from Favara Grande indicated methane consumption values of up to 59.2 nmol g−1 soil d.w. h−1. One of the three sites, FAV2, where the highest oxidation rate was detected, was further analysed on a vertical soil profile, the maximum methane consumption was measured in the topsoil layer, and values greater than 6.23 nmol g−1 h−1 were still detected up to a depth of 13 cm. The highest consumption rate was measured at 37 C, but a still detectable consumption at 80 C (>1.25 nmol g−1 h−1) was recorded. The soil total DNA extracted from the three samples was probed by Polymerase Chain Reaction (PCR) using standard proteobacterial primers and newly designed verrucomicrobial primers, targeting the unique methane monooxygenase gene pmoA; the presence of methanotrophs was detected at sites FAV2 and FAV3, but not at FAV1, where harsher chemical–physical conditions and negligible methane oxidation were detected. The pmoA gene libraries from the most active site (FAV2) pointed to a high diversity of gammaproteobacterial methanotrophs, distantly related to Methylocaldum-Metylococcus genera, and the presence of the newly discovered acido-thermophilic Verrucomicrobia methanotrophs. Alphaproteobacteria of the genus Methylocystis were isolated from enrichment cultures under a methane-containing atmosphere at 37 C. The isolates grow at a pH range of 3.5 to 8 and temperatures of 18–45 C, and consume 160 nmol of CH4 h−1 mL−1 of culture. Soils from Favara Grande showed the largest diversity of methanotrophic bacteria detected until now in a geothermal soil. While methanotrophic Verrucomicrobia are reported as dominating highly acidic geothermal sites, our results suggest that slightly acidic soils, in high-enthalpy geothermal systems, host a more diverse group of both culturable and uncultivated methanotrophs.en
dc.relation.ispartofBiogeosciences (BG)en
dc.relation.ispartofseries/11 (2014)en
dc.subjectgeothermal soilsen
dc.subjectmethanotrophic activityen
dc.subjectgeothermal gasesen
dc.titleMethanotrophic activity and diversity of methanotrophs in volcanic geothermal soils at Pantelleria (Italy)en
dc.subject.INGV01. Atmosphere::01.01. Atmosphere::01.01.02. Climateen
dc.subject.INGV01. Atmosphere::01.01. Atmosphere::01.01.07. Volcanic effectsen
dc.description.obiettivoSpecifico4V. Vulcani e ambienteen
dc.description.journalTypeJCR Journalen
dc.contributor.authorGagliano, A. L.en
dc.contributor.authorD'Alessandro, W.en
dc.contributor.authorTagliavia, M.en
dc.contributor.authorParello, F.en
dc.contributor.authorQuatrini, P.en
dc.contributor.departmentUniversità di Palermo, Dip. DiSTeMen
dc.contributor.departmentIstituto Nazionale di Geofisica e Vulcanologia, Sezione Palermo, Palermo, Italiaen
dc.contributor.departmentUniversità di Palermo, Dip. STEBICEFen
dc.contributor.departmentUniversità di Palermo, Dip. STEBICEFen
item.fulltextWith Fulltext-
item.openairecristype Nazionale di Geofisica e Vulcanologia (INGV), Sezione Palermo, Palermo, Italia- Nazionale di Geofisica e Vulcanologia (INGV), Sezione Palermo, Palermo, Italia-à di Palermo, DiSTeM, Italy-à di Palermo- Nazionale di Geofisica e Vulcanologia- Nazionale di Geofisica e Vulcanologia-
crisitem.department.parentorgIstituto Nazionale di Geofisica e Vulcanologia-
crisitem.classification.parent01. Atmosphere-
crisitem.classification.parent01. Atmosphere-
Appears in Collections:Article published / in press
Files in This Item:
File Description SizeFormat
BioGeoSci.Gagliano&al.pdfmain article2.03 MBAdobe PDFView/Open
Show simple item record


checked on Feb 10, 2021

Page view(s) 5

checked on Jun 28, 2022

Download(s) 50

checked on Jun 28, 2022

Google ScholarTM